Anti Inflammed Activity Test of Extract, Water Fraction, Ethyl Acetate, and N-Hexan Sendok Leaf (Plantago Major L.) to Red Blood Cell Membrane Stability

Abstract

Plantago major L or leaf spoon has been recognized
by the Indonesian people as a traditional medicinal plant
which has anti-inflammatory properties. The leaf spoon
has antioxidant activity which acts as anti-inflammatory
by capturing free radicals. This study aims to determine
the content of secondary metabolites and to determine
anti-inflammatory activity in vitro with the HRBC (Human
Red Blood Cell) membrane stabilization method. Red
blood cell membrane stabilization has been used as a
method to determine anti-inflammatory activity in vitro.
The concentrations of each extract and spoon leaf fraction
were 4000 ppm, 2000 ppm, 1000 ppm, 500 ppm and
Positive control used diclofenac sodium, which is a NSAID
drug class (Non Steroid Antiinflammatory). Diclofenac
sodium as a standard drug at a concentration of 100 ppm
is capable of stabilizing red blood cells by 91.56%.This
is because the red blood cell membrane is similar to the
lysosome membrane (Shenoy et al., 2010). The results of
IC50 extract, water fraction, ethyl acetate, and n-hexane
were 167.282 ppm, 20.89 ppm, 14.78 ppm, 15.86 ppm