Anti Inflammed Activity Test of Extract, Water Fraction, Ethyl Acetate, and N-Hexan Sendok Leaf (Plantago Major L.) to Red Blood Cell Membrane Stability

Dewi Maha Putri Kezia, Wardani Siska Tatiana, Setianto Rony, Septiarini Dwi Anita, Veranita Weri, Mahardika Putra Muladi


Plantago major L or leaf spoon has been recognized
by the Indonesian people as a traditional medicinal plant
which has anti-inflammatory properties. The leaf spoon
has antioxidant activity which acts as anti-inflammatory
by capturing free radicals. This study aims to determine
the content of secondary metabolites and to determine
anti-inflammatory activity in vitro with the HRBC (Human
Red Blood Cell) membrane stabilization method. Red
blood cell membrane stabilization has been used as a
method to determine anti-inflammatory activity in vitro.
The concentrations of each extract and spoon leaf fraction
were 4000 ppm, 2000 ppm, 1000 ppm, 500 ppm and
Positive control used diclofenac sodium, which is a NSAID
drug class (Non Steroid Antiinflammatory). Diclofenac
sodium as a standard drug at a concentration of 100 ppm
is capable of stabilizing red blood cells by 91.56%.This
is because the red blood cell membrane is similar to the
lysosome membrane (Shenoy et al., 2010). The results of
IC50 extract, water fraction, ethyl acetate, and n-hexane
were 167.282 ppm, 20.89 ppm, 14.78 ppm, 15.86 ppm

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